Last data update: May 06, 2024. (Total: 46732 publications since 2009)
Records 1-3 (of 3 Records) |
Query Trace: Le NT[original query] |
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Zika preparedness and response in Viet Nam
Nguyen DT , Do HT , Le HX , Le NT , Vien MQ , Nguyen TB , Phan LT , Nguyen TV , Luong QC , Phan HC , Diep HT , Pham QD , Nguyen TV , Huynh LK , Nguyen DC , Pham HT , Ly KK , Tran HN , Tran PD , Dang TQ , Pham H , Vu LN , Mounts A , Balajee SA , Nolen LD . Western Pac Surveill Response J 2018 9 (2) 1-3 The findings and conclusions in this article are those of the authors and do not necessarily represent the official positions of the United States Centers for Disease Control and Prevention. |
Disparate detection outcomes for anti-HCV IgG and HCV RNA in dried blood spots.
Tejada-Strop A , Drobeniuc J , Mixson-Hayden T , Forbi JC , Le NT , Li L , Mei J , Terrault N , Kamili S . J Virol Methods 2014 212c 66-70 Dried blood spots (DBS) expedite the collection, storage and shipping of blood samples, thereby facilitating large-scale serologic studies. We evaluated the sensitivity of anti-HCV IgG testing and HCV-RNA quantitation using freshly prepared and stored DBS derived from HCV-infected patients. Protocols for elution were optimized using DBS prepared from plasma of 52 HCV-infected persons and 51 uninfected persons (control DBS), then applied to DBS from 33 chronic hepatitis C patients that had been stored at -20 degrees C for 5 years (stored DBS). Control and stored DBS, and their corresponding plasma, were processed for anti-HCV IgG testing using the VITROS chemiluminescence assay (CIA) and the HCV 3.0 enzyme immunoassay (EIA) (Ortho-Clinical Diagnostics), and for HCV RNA quantitation by quantitative (q) RT-PCR. HCV genotyping was conducted by nucleotide sequencing. The sensitivity of CIA and EIA in control DBS was 92% and 90%, respectively, compared to 100% and 97%, respectively, in stored DBS. The sensitivity of HCV RNA detection was 88% in control DBS, compared to 36% in stored DBS. Specificity was 100% for all the assays in both control and stored DBS. Genotypes 1, 2 and 3 were detected in 16 (62%), 6 (23.1%), and 4 (15.3%) samples, respectively. Sequences generated from DBS and their corresponding plasma samples were identical. Whereas the sensitivity of anti-HCV IgG detection in stored DBS was equivalent to that in recently prepared DBS, the sensitivity of HCV RNA detection was markedly lower in stored DBS compared to recently prepared DBS. Stored DBS may be reliably used for anti-HCV detection but for HCV-RNA-based testing freshly prepared DBS is preferable to stored DBS. |
Laboratory-based surveillance for hepatitis E virus infection, United States, 2005-2012
Drobeniuc J , Greene-Montfort T , Le NT , Mixson-Hayden TR , Ganova-Raeva L , Dong C , Novak RT , Sharapov UM , Tohme RA , Teshale E , Kamili S , Teo CG . Emerg Infect Dis 2013 19 (2) 218-22 To investigate characteristics of hepatitis E cases in the United States, we tested samples from persons seronegative for acute hepatitis A and B whose clinical specimens were referred to the Centers for Disease Control and Prevention during June 2005-March 2012 for hepatitis E virus (HEV) testing. We found that 26 (17%) of 154 persons tested had hepatitis E. Of these, 15 had not recently traveled abroad (nontravelers), and 11 had (travelers). Compared with travelers, nontravelers were older (median 61 vs. 32 years of age) and more likely to be anicteric (53% vs. 8%); the nontraveler group also had fewer persons of South Asian ethnicity (7% vs. 73%) and more solid-organ transplant recipients (47% vs. 0). HEV genotype 3 was characterized from 8 nontravelers and genotypes 1 or 4 from 4 travelers. Clinicians should consider HEV infection in the differential diagnosis of hepatitis, regardless of patient travel history. |
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